Major outer membrane proteins of Prochlorothrix hollandica (Prochloraceae)

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ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

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isolation and purification of major outer membrane proteins from brucella abortus s-99

isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1990

ISSN: 0378-1097,1574-6968

DOI: 10.1111/j.1574-6968.1990.tb13964.x